Reliable Life Cycle Assessment (LCA) indicators for wastewater treatment plants (WWTP) construction and operation phases are still a demand mainly in developing countries. Thus, the purpose of this paper was to present and discuss the environmental performance of a full-scale WWTP installed in a Brazilian city using LCA approach. The treatment process consists of a UASB reactor followed by constructed wetlands, which makes it particularly attractive to developing countries due to its operational simplicity. The Life Cycle Inventory (LCI) was developed from a WWTP design and operation data including those of untreated wastewater and effluent quality. The results show that the environmental impacts from construction phase should not be neglected in LCA studies of low complexity treatment technologies (e.g. UASB reactor, constructed wetlands and pond systems). There is a trade-off between the use of materials and energy for construction and the low energy and materials consumption during the operation phase in these systems. The majority share of hydroelectric generation in the energy matrix and the combination of anaerobic and natural processes for wastewater treatment have contributed to a smaller impact potential for the operation phase. The LCA approach should be associated with plans and actions to face the challenges of providing wastewater treatment in developing countries. Only in this way, compliance with the eco-efficiency targets and protect public health will be guaranteed. V.The toxic effects of multi-nanomaterial systems are receiving increasing attention owing to their inevitable release of various nanomaterials. Knowledge of the bioavailability of the new carbon material ball-milled biochar (BMB) and its synergistic toxicity with metal oxide nanoparticles in bacteria is currently limited. In this study, the interactions of BMB with copper oxide nanoparticles (CuO NPs) and their synergistic toxicity towards Streptomyces coelicolor M145 were analyzed. Results showed that the cytotoxicity, ROS level and permeability of cells changed greatly with the pyrolysis temperatures of biochar and the concentrations of CuO NPs. The greatest cytotoxicity (up to 63.1%) was achieved by adding 20&nbsp;mg/L CuO NPs to BMB700. The ROS level and cell permeability of this treatment was also the highest, about 4.2 folds and 2.9 folds greater than that of control, respectively. The combination of 10&nbsp;mg/L BMB700 with 10&nbsp;mg/L CuO NPs can maximize production of antibiotics, with the yield of undecylprodigiosin (RED) and actinorhodin (ACT) 3.0 times and 4.2 times higher than that in the control, respectively, and the change trend of related genes was consistent with that of antibiotics production. Mechanism analysis showed that the different adsorption capacity of BMB of different pyrolysis temperatures on copper ions played a vital role in the synergistic toxicity, and the increase in cell membrane permeability caused by cell collisions with particles was also an important reason for cytotoxicity. https://www.selleckchem.com/products/rottlerin.html Overall, the synergistic toxicity of BMB with other NPs varies the pyrolysis temperatures, when considering the synergistic toxicity of these materials, the preparation conditions need to be taken into account so as to assess their environmental risks more accurately. On the other hand, this research may provide a new approach for the antibiotic industry to increase its output. The energy-water nexus is a concept widely established but rarely applied to product and, in particular, to food and beverage products, which have a great influence on greenhouse gases emissions. The proposed method considers the main nexus aspects in addition to other relevant aspects such as climate change, which is deeply linked with energy and water systems, and assessing process as well as product. In this framework, this study develops an integrated index (IWECN) that combines life cycle assessment (LCA) and linear programming (LP) to assess energetic, water and climate systems, enabling the identification of those products with minors energetic and water intensity and climate change effects and helping to the decision-making process and to the development of eco-innovation measures. In this case, the product assessed was one bottle (70&nbsp;cl) of gin and two main hotspots were identified the production of the glass bottle and the energy requirements of the distillation stage. Based on that, several eco-innovation strategies were proposed the use of photovoltaic solar energy as energy source and the substitution of the glass bottle by a plastic one and by a tetra brick. The nexus results indicated that the use of solar photovoltaic energy and plastic as bottle material was the best alternative decreasing 58% the IWECN value of the production of one bottle of gin. The sensitivity analysis presented a strong preference for photovoltaic solar energy in comparison with electric power and for the reduction of the glass bottle weight or its substitution by a plastic bottle. The use of the IWECN index is extendable to any product with the aim of facilitating the decision-making process in the development of more sustainable products to introduce them in new green markets. Flood risk can be reduced at various stages of the disaster management cycle. Traditionally, permanent infrastructure is used for flood prevention, while residual risk is managed with emergency measures that are triggered by forecasts. Advances in flood forecasting hold promise for a more prominent role to forecast-based measures. In this study, we present a methodology that compares permanent with forecast-based flood-prevention measures. On the basis of this methodology, we demonstrate how operational decision-makers can select between acting against frequent low-impact, and rare high-impact events. Through a hypothetical example, we describe a number of decision scenarios using flood risk indicators for Chikwawa, Malawi, and modelled and forecasted discharge data from 1997 to 2018. The results indicate that the choice between permanent and temporary measures is affected by the cost of measures, climatological flood risk, and forecast ability to produce accurate flood warnings. Temporary measures are likely to be more cost-effective than permanent measures when the probability of flooding is low.